Below is our D29 phage DNA, cut with the BSTE II enzyme1. We also cut our new phage DNA with this enzyme, yielding very interesting results regarding how the phages are related to each other. See the Cla 1 digestion, also, with respect to Restriction Fragment Length Polymorphisms (RFLP) of the phage DNA. The DNA marker is 1Kb DNA ladder, and the sizes of the DNA bands are indicated on the figure. Here is a question for students who have learned how to use the Clone program, or are at least familiar with expected product molecular weight as a result of using this program: Has the D29 genome, after digestion, yielded the expected restriction fragment length polymorphisms? The expected fragment sizes can be found using the NIH's published genome data.
[Do: insert expected fragment sizes.] [Insert scanned DNA marker.] [hyperlink NIH web-site].
We would have posted more about our DNA analysis results (it is REALLY interesting to compare all the DNA from a group of new phage as we did) online, but we have found out that scientific publication copyright on certain scientific journals (PDF file, search for the word "web" within) restricts us from this.