Below is our phage DNA, cut with the Cla I enzyme.  This yields a restriction fragment length polymorphism (RFLP) of the different phage.  Note the differences in numbers of restriction sites that are apparent, each genome was cut in a different pattern.  The DNA marker is 1Kb DNA ladder, and the sizes of the DNA bands are indicated on the figure.  Our concern: the D29 phage was predicted to cut with this Enzyme, it did not.  Therefore, we will repeat the experiment.  We were able to use this experimentation to draw distinctions and relationships between our different phage.  These phage DNAs will be cut again, and run side by side because of their similarities.  If these phages turn out to be related but different, or, if there are any phage isolated from different soil samples with the same RFLP, there is a very interesting story here with respect to phage biodiversity.

We would have posted more about our DNA analysis results (it is REALLY interesting to compare all the DNA from a group of new phage as we did) online, but we have found out that the scientific publication copyright on certain scientific journals (PDF file, search for the word "web" within) restricts us from this.