Day 11 thoughts.  Phagefinders. 

Planned:  D29 10-3 are in the incubator, and we have our harvested D29.  The 10-3 D29 need to be looked at 10 AM when Bill arrives.  We want to check our new samples in order to propagate ASAP.  Our parafilm plates on the side of the room, growing at RT, may be ready for harvest on Monday.  What is going on with Stephen's phage? And other phages that have been picked and extracted from buffer?  We also have a Smeg plate in the drawer and we are asking if Smeg is affected by light.  We have smeg cultures in the shaker room that we need to look at from our single colony, and we also have soil samples that have incubated over the weekend.  These samples have been incubated for a longer duration in hope to extract phage.  Kind of a one shot deal.  We just plated our new phages, hopefully we have plaques on monday for picking and plating further.  We also harvested D29.  We also need to look at the rhizopus plates, perhaps they are ready to put at RT. Chiara wants to look at the growth that we plated on tables, and see if these can be grown in media or if media kills them, and if it grows, let's try to find a phage for these. Lazlo needs to plate. 

Did: 

Students were given block partners; so they could track samples better. 

Order:

we photographed Kristen's phage and our phage resistant mutants.

Students had different things to do today:

Chiara: plated new samples that incubated over the weekend, we didn't have virus yet.

    Chiara is plating her Candida sample.  She is filtering.

    She began incubating the water sample fountain sample, too. 

Lazlo: plated new samples, didn't have virus yet. 

    He plated 2 samples that we suspect will have stuff in it.

Amanda: plated sample 2, started 2 new samples.

Assumption: 2x10^8 smegmatis in 1 mL media.  We want to have 1000 mycobacteria in 10 mL media.  To calculate, Amanda will do the math on a sheet of paper and then tell me how to do this dilution. 

Genevieve: new student, helped Kristen and also started new samples.

    Genevieve will also dilute out Kristen's compost heap sample. 

Stephen: His phage was not apparent on plate, we are wondering if the phage has become lysogenic.  He is diluting from a HT preparation made the week before, plating -4,-5,-6,-7,-8,-9,-10.  He will make new HT and find a new plaque for picking from this preparation.  Also, he will be monitoring when plaques arise and disappear on the plates. 

Chris: didn't have plaques after picking a plaque. 

    1 plate is incubating at RT, the other is at 37 C.

    Chris will pick 3 plaques, and will dilute and plate them at -1, -2, -3, -4, -5.

    Thinking: dilution phenomena since his is very lysogenic.

    Chris is also replating his phage that didn't plaque. 

He is also contributing to group via collecting D29 mutation data. 

Table to make:

Student:    # mutant colonies:    age of Smeg.:

Kristen: phage A has new plaques.  She will calcuate the #. = dilution value.

she will pick a plaque and will dilute as before and plate -1, -2, and -3.

phage B has funny smeg, no plaques.  She will redo 1 colony from the original and plate -1, -2,. -3, -4 and -5.  On another colony, she will plate -1, -2, -3.  (Different smeg). 

phage C has some plaques, but funny smeg.  Counted 10-1, 10-0.  Pick 2 plaques, and dilute

Blake:  Is making HT of his phage WJ, which is from Bill's soil sample next year. 

Priorities, any new phage?  smeg cultures in the room, our new soil samples. 

Did: 

1) dilution plate our new phage samples. 

2) dilution plate Steve's phage using independent dilutions...we will collect a data set as we did for D29. 

Bill questions: when go to the zoo? 

Today's materials:   

Pointers: