Day 14 thoughts.  Phagefinders. 

Planned:  Dilution of smeg sample over the weekend, for Monday.  Under-dilute samples of smeg are with us today, and tomorrows plans are to take a field trip with Dr. Jacobs.  These cultures will have enough smeg in them to plate today.  This may  complete the Lamarck vrs. Darwin experiment.  We will begin by calculating the number of bacteria we anticipate to have in solution, and will plate not a lawn, but for colonies.  I don't know if this will work, but we will have results that can persist over the weekend.  Ideal: plate with 100 colonies, another with 1000 colonies, another with 10,000 colonies expected if the phage doesn't wipe out.  Next week, we will take the same samples and will plate D29, trying to make the equivalent of a lacy plate if we do not have a valid result.   

However, we also need to have phage prepped for EM studies, Monday. 

Amanda: New phage? It has been 2 days.  Also, how is the 1 d old samples?  Amanda accepted new phage. 

Kristen: it has only been 1 day since many samples were prepared, probably too early.  Plates will have to be set out or placed in the fridge tomorrow.  Today, we must identify which samples will be set out, tomorrow.  These samples will be written on a sheet of paper and set aside within the incubator, etc.    Kristen needs to harvest phage today, where she did not have time to do so the day before. 

Kristen is making this table today, for her samples:

Sample Acronym:    Filial Purity:    HT?        Other phage seen at this time? 

Chris:  Chris picked plaques yesterday, photographed his samples, and plated yesterday.  Chris also has a really lysogenic sample that comes from under the bridge.  This sample needs to be picked after photography today.  The plate then needs to be stored.  Chris also has phage from the creek soil.  Creek sample will be picked today, even though it's light, and Kristen will remove and place in Fridge tomorrow. 

Lazlo:  Although only 1 day, how do samples look?  Lazlo has a new sample for us to incubate today, Airshaft.  Also may a plaque on sample BF16.  He will pick this plaque today.  Lazlo also has 2 plates that we can't tell yet.  Kristen will remove these from the incubator tomorrow.  Lazlo is also starting another soil sample today. 

Stephen:  Do we have new plaques?  Pick and plate if so.  The new phage has made a lacy plate.  Therefore, pick and plate the phage after diluting...plate -2, -4, -6, -7.  Then, harvest the lacy plate.  This will be F1 plating.  S17 is this phage.  Another phage:  S8, appears to have 2 types of plaques.  This plate had a plaque picked from a clear plaque, and plated from -1 unto -5.  Therefore, today he will pick a turbid plaque and dilute and plate, -1, -3, -5.  S5 -1, offered to give away, also has plaques...I recommend that you set sample S5 aside for another day, parafilm it, and put in fridge. 

Blake:  How did it go with isolating phage DNA? An advanced procedure.  Show us data, great job explaining the procedures to the group yesterday.  Blake is with us today, of 8 DNA preps, only 1 worked.  .8% LE sea? Agar. 

Reid: 

Genevieve:  Although only 1 day, how do samples look?  Genevieve has a new phage today, although the plate is light.  This plate will appear lacy tomorrow, so put this into the incubator unto tomorrow.  On Monday, we may harvevst the plate for EM.  Genevieve really wants to plate the water today, from the raindrop.  She's worried that phage age has an effect. 

Chiara: new samplel today.  Her Drinking sample fountain will incubate for one additional day. We also have some possible plaques, from Canada, if they are or are not we aren't sure because if it is, the phage is extremely lysogenic...very very turbid.  Chihara is picking 2 plaques today, diluting 10^0-->10-4, plating all of these. 

Amanda:  Took new sample, from under bridge, highly lysogenic sample, is plating this today. 

Kristen: is plating today, CH1 is in round 2 plating today.  She will need to store this plate tomorrow, leave at RT over the weekend.  She is also harvesting C's lacy plate, = F2, this will go EM on Monday.  She also picked a plaque from the original plating of Phage A, this will become a new generation of A.  Tomorrow, long weekend, this plate should be set at RT. 

Did: 

Check and ensure we have segrergated which plates need to come out of the incubator tomorrow. 

Remove Smegmatis today if isn't a plan to do this tomorrow via Kristen. 

Today's materials:   

Pointers:

Next year, tracking of student individual whereabouts should be done by the students--it take the instructor lot's of time to follow where the student research is.  Although I follow the student notebook and comments, which are consistent and accurate, I plan to rely entirely on this information without my tracking it next year.  This means I will spend more time in the student notebooks.