Day 19 thoughts.  Phagefinders. 

Planned: need to autoclave tips, plate mutant smeg with non-mutant smeg on D-29 (3 students), Steve, which phage samples made phage? 

Cell paper reading, clone program discussion, bill in at noon for lunch, plates, picture-pages, smegmatis. 

Find where this data needs to go:

D29 mutation rate:        7/22/03 collection.

Student:        # mutant colonies:            type of smeg:

Lazlo                        80                            new

Amanda                    52                            new

Stephen                    650                           old

Chris                        552                            old

Kristen                     450                            new

Did:  Regarding the Lamarcking vrs. Darwinian theory experimentation,

Chris: new phage from house samples.  he has picked this today and is going with this. 

Amanda: no phage, although she had before. 

Chiara: these are the results of her plating phage CKCA (lucy) for F2: 10-0 has a giant plaque and confluent smeg that is similar to the smeg on all other dilutions, grown at 37C.  10-1 has a slightly smaller giant plaque, with lots of tiny plaques in smeg lawn.  10-2 there are 2 different plaques but Reid thinks they are the same plaque, all are small.  10-3 has about 10 fold less of these, so the dilution of the new phage phenotype is apparent. 

Chiara will do 3 things: will pick from the F1 again, and will plate out the same dilutions.  She will then also pick from the big huge plaque at F2 10^0, and will dilute this out.  Also, will pick from 10^-2 F2, and will dilute.  Note that the plaques on the -2 diliution are bigger than on the F1.  It is important to note that Chiara had combined 2 plaques from the F1 when she picked.  However, I maintain...phage-phage interactions on the smeg are really interesting.  I think the plaques are suppressing each other when nearby, somehow.  With many phage,

Discussion of clone program:

Today's materials:   

Pointers: