Day 25 thoughts.  Phagefinders. 

Planned:  How do plaque forming units (PFU),  as determined by spot dilution,  relate to the amount of DNA that we isolate?  We will also top-agar plate rhodococcus today (sideline experiment, hunting for phage of another bacteria), at 2 different concentrations.  This will require filtering of dirt incubated with rhodococcus for 48 h, performing the isolating phage procedure, using rhodococcus instead of smegmatis. 

AM: make rhodococcus top-agar, using LB with Noble Agar added. 

Where are my F2 phage samples for EM? 

Let's look at the DNA results from yesterday, while we do another gel analysis today. 

Did: 

Digestion:    Cla 1 restriction digestion.  Chris, Blake:  ran gel, 0.8% agarose. 

Paragraphs:  Bste II restriction enzyme analysis, emailed to me.  including a conversation with Lazlo. 

                        Have we found unique phage fingerprints?

                        Did we find unique phage?

                        Describe the actual Molecular Weight of your fragments, after BSTE II restriction.

Definitions:    Students generated definitions for the phagefinders vocabulary list.

Prep of phage for EM:  AECOM analytical imaging does the grid preparations for us, we give them aliquots of our phage. 

Today's materials:   

Pointers:  There is down-time during the DNA digestion stages.