
This is Genevieve's composition (and Image) about Electron Microscopy:

1) An electron source (ex. a cathode) sends electrons downward from the top of the transmission electron microscope (TEM). Solenoids and/or an area of positive charge at the bottom of the TEM focus and accelerate the beam downward. (Down is a good direction so gravity is not an obstacle.) 2) Electrons pass through the grid and specimen, but some electrons are stopped by the "opaque" parts of the specimen, which has been stained with phosphotungstic acid for this effect. Usually, specimens are too permeable to electrons without acid and so would not produce a good image. The result is a negative image of the specimen. 3) After the beam hits the specimen and grid, it spreads out (the image is magnified) until it hits a stage at the bottom of the TEM tube; the stage is coated with a substance that fluoresces when hit with a charged particle, so the electrons that passed through the more translucent parts of the specimen help form an image on the stage. 4) This image can be further magnified by a modified simple light microscope, whose light source is the stage itself. A camera is also attached. |