Look into NEB website, find out what organisms BSTeI and ClaI come from, the hint is it starts with a c... could be coronabacteria, or chlorstridia...

Bill asked how small the phages were believed to be...50,000 times...and we saw 2 or 3

Bacillus stereothermophilus...Caryophanon latum L. 

Genevieve's plaques were of different sizes, and she believes that temperature changes could be effective.  This is a constant concern for us...Bill says that plaque size is variable as a function of smegmatis density.  We could concentrate smeg, using a centrifuge 10 fold...say we have 100 plaques, is 10 times vrs. 5 or 1 x times the amount of cells, plaque sizes will change.  Who will have the largest plaques?  Since it is a higher concentration, Bill had student's vote on plaque size...some student explanation...Blake thinks it would be larger with more phage.  Bill-why does the plaque stop growing?  Chiara: phage runs out of food.  Bill: bacteria have reached saturation, they have gone into a stationary phase, and have chilled out.  What is happening, the reason why it stops, is that the bacteria have entered stationary phase.  Bill drew a growth curve of bacteria, explaining stationary, logarithmic growth.  If cells are in the stationary phase, plaques will be smaller. 

Bill liked Steve's phage, and believed that

Does eveyone know the smell of smeg cultures now?  The way I could tell if it wasn't contamination was smell.  Cool phages.  In barnyard, it has a gene that is homologous to m.lycococcusluteus gene called recession factor.  It tells cell to stay in quiescent state until it comes into contact with this protein, and then it takes off.  The reality is that we know so little...we have used just a couple phages over all the course of studies before.  Maybe we need to think about many biological things in new ways.  I am really intrigued by the halos that you have.  In certain steptomyces strains, these have been described and the mechanisms have not been.  One of the things we desperately need concerns that when MTB goes into a dorminant stage, nothing kills it.  If we had a phage that kills dormant bugs, it would tell us that drugs could be made that kill dormant drugs.  We don't have drugs that do this.  Your results are very stimulating to me...

Bill talked about the clone program, and how we could recombine 2 different phages together with restriction enzymes, most of your cloning is done on the computer first, then they do the experiment. 

Steve: so, can you really take any gene sequence and connect it with another?  Bill: some gene sequences could be a toxic event to the cell.  Many things aren't allowed by nature, but theorretical...one of my friends made a plant, they sense the wind and turn on different sets of gene.  He hooked up lucierase, and when the plant is stroked, it glows.