Student paragraphs 5

One can easily tell that the phages are all unique as they all had different banding patterns from the BstEII cut. The restriction enzyme recognizes a sequence of bases and cuts the DNA at these sites. As long as the DNA is different, the restriction enzyme will cut it into fragments of different lengths. Phages 33, e2 and 17 have a similar bands close to the wells. The fact they are close to the wells means they are large fragments and in relations to the ladder they are over 12,000 bp's. The fact that they are dark notes that there are many bands on that length/near piled up near each other. Both of these together could indicate the DNA didn't cut, or didn't cut well, as there Arnet any lighter bandings future down (if so they are very faint). 1 is similar to 27, except 1 has more bands, and therefore more cuts. 11 is somewhat similar to 39 however 11 has more bands. G1 and F don't look anything like the others. G1's first two bands are like d29's- only lighter. g1 and F are both Blake's and very different I remember him saying, so that is a reasonable correlation. I have no idea of the phages and their respective plaque morphologies, so the relations in DNA fragments cannot be connected to know phage traits.

Schwebach's comments: soon, we will be connecting plaque morphologies to restriction digestion patterns.

Killah (sample 39) was cut with BSTE II, a restriction enzyme and run on a gel. The first band is more than 12,000 Daltons, the next band is about 8,000 Daltons, and the last is at about 5,000 Daltons. It is most similar to sample number 11. This is a unique phage fingerprint, thus Killah is a unique, new phage.

We have found unique phage fingerprints. I compared the restriction digestions from BSTE II for my phages to all the others, both old and new. My phage fingerprints are extremely similar even though they are from completely different areas. They show some similarities to CHE 8, Omega and Barnyard. After studying this, I conclude that these are new phage. The molecular weights of some of the more prevalent bands are 5000 daltons, 3000 daltons,1650 daltons and 1000 daltons.

BF-03-16: BF-03-16 did not show up in the cut with BstEII, which means one of two things. The first of which is that there was no restriction sites, meaning that the GGTNACC did not appear in the sequence. The second possibility is that the cut failed. Due to the possibility of the second event, it would be a good idea to re-cut, but because of the time restraints.

BSTe II Results Analysis When my two phage samples were cut by BSTe II the results were opposite from each other. The enzyme absolutely shredded the DNA of the ø S5. This shows that there were many cutting sites on the strand. This phage definitely is unique and its strand reacted to the enzyme in a similar manner to Bxz-1. It contained above 12,000 base pairs. In the DNA of ø S17 the enzyme had no effect on the strand. If this is not due to a major experimental error, this is something which has never been seen before and requires further study.